Rat BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit – E-EL-R0128

Rat BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit

The BRAK ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BRAK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for BRAK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BRAK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BRAK. You can calculate the concentration of BRAK in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Breast And Kidney Expressed Chemokine

Abbreviation : BRAK

Uniprot ID : Q8K453

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 56.25 pg/ml

Detection Range : 93.75 – 6000 pg/ml

Manual & Datasheet

More information about this Rat BRAK (Breast And Kidney Expressed Chemokine) ELISA Kit from Elabscience