Rat BH4 (Tetrahydrobiopterin) ELISA Kit – E-EL-R2559

Rat BH4 (Tetrahydrobiopterin) ELISA Kit

The BH4 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Rat BH4. During the reaction, Rat BH4 in the sample or standard competes with a fixed amount of Rat BH4 on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Rat BH4. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BH4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Tetrahydrobiopterin

Abbreviation : BH4

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

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