Rat ASM (Acid Sphingomyelinase) ELISA Kit – E-EL-R1141

Rat ASM (Acid Sphingomyelinase) ELISA Kit

The ASM ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ASM. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ASM and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ASM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ASM. You can calculate the concentration of Rat ASM in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Acid Sphingomyelinase

Abbreviation : ASM

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

More information about this Rat ASM (Acid Sphingomyelinase) ELISA Kit from Elabscience