Rat APLN (Apelin) ELISA Kit – E-EL-R2413

Rat APLN (Apelin) ELISA Kit

The APLN ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Rat APLN. During the reaction, Rat APLN in the sample or standard competes with a fixed amount of Rat APLN on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Rat APLN. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat APLN in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Apelin

Abbreviation : APLN

Uniprot ID : Q9R0R3

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Rat APLN (Apelin) ELISA Kit from Elabscience