Rat ANXA5 (Annexin A5) ELISA Kit – E-EL-R2391

Rat ANXA5 (Annexin A5) ELISA Kit

The ANXA5 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ANXA5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ANXA5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ANXA5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ANXA5. You can calculate the concentration of Rat ANXA5 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Annexin A5

Abbreviation : ANXA5

Uniprot ID : P14668

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Rat ANXA5 (Annexin A5) ELISA Kit from Elabscience