Rat ANG II (Angiotensin II) ELISA Kit – E-EL-R1430

Rat ANG II (Angiotensin II) ELISA Kit

The ANGII ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Rat ANG II. During the reaction, Rat ANG II in the sample or standard competes with a fixed amount of Rat ANG II on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Rat ANG II. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat ANG II in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Angiotensin II

Abbreviation : ANGII

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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