Rat β-EP (Beta-Endorphin) ELISA Kit
The β-EP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with β-EP. During the reaction, Rat β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat β-EP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat β-EP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<
Detected Target name: Rat Beta-Endorphin
Abbreviation : β-EP
Format : 96 rx
Reactivity : Rat
ELISA configuration : Competitive
Sensitivity : 9.375 pg/ml
Detection Range : 15.625 – 1000 pg/ml
More information about this Rat β-EP (Beta-Endorphin) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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