Pregnenolone ELISA Kit – E-EL-0086

Pregnenolone ELISA Kit

The ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Pregnenolone. During the reaction, Pregnenolone in the sample or standard competes with a fixed amount of Pregnenolone on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Pregnenolone. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pregnenolone in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

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Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,469

Detection Range : 0.781-50 ng/mL

More information about this Pregnenolone ELISA Kit from Elabscience