Neuron Specific Enolase Antibody – RPCA-NSE

Encor Rabbit polyclonal to Neuron Specific Enolase (NSE) 

Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Perhaps not surprisingly since neurons require a great deal of energy they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).

HGNC name(s) : ENO2

Host : Rabbit

Clonality : Polyclonal

ID : EnCor Biotechnology Neuron Specific Enolase NSE

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG

Conjugation : none

Immunogen : Recombinant full length human

Mass of detected protein : 47 kDa

Uniprot ID : P09104

KGNC name : ENO2

RRID # : AB_2277965

Purification : Serum

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions: 

WB: 1:2 000. ICC/IF and IHC: 1:500.

Datasheet

Link to on EnCor’s website 

References :

1. Shaw, G . Morse, S., Ararat, M. and Graham, F. Transformation of human cells by human adenoviruses and the origins of HEK 293 cells. Faseb Journal 16:869-871 (2002).

2. Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006).