Tubulin Antibody – MCA-1B12

Encor Mouse Monoclonal to beta tubulin

Tubulins are a major class of cytoskeletal proteins and divided into five distinct classes, namely α, β, γ, δ and ε tubulins. The most abundant members of the tubulin family are the α and β-tubulins and are the major components of cytoplasmic microtubules. The various subunits have molecular weights of approximately 55 kDa and are 50% identical to one another at the protein sequence level. Microtubules are assembled from stable dimer of one α and one β subunit, and polymerization from dimers to assembled microtubules requires GTP. Microtubules are involved in a number of essential cellular functions including the maintenance of cell shape, transport, motility, cell signaling and mitosis (1). β tubulin is regarded as a “house keeping” protein which is generally not altered much in expression as a result of experimental manipulations. As a result antibodies to β tubulin are widely used as loading controls in western blotting as a standard by which the levels of other proteins may be measured. The important role of microtubules in cell division makes them a desirable target for the development of chemotherapeutic agents directed against rapidly dividing cancer cells (2). MCA-1B12 was raised against tubulin purified from pig brain and reacted with recombinant β-tubulin (Abcam), but not recombinant α-tubulin (Abnova) by ELISA and dot blots.

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HGNC name(s) : TUBB

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Host : Mouse

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Clonality : Monoclonal

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ID : EnCor Biotechnology Tubulin 1B12

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Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Isotype : IgG2b

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Conjugation : none

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Immunogen : Purified bovine

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Mass of detected protein : 55 kDa

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Uniprot ID : P07437

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KGNC name : TUBB

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RRID # : AB_2572389

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Purification : Affinity purified at 1 mg/mL

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Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

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Validated applications : WB | IF/ICC | IHC

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Suggested Dilutions:

Western blot : 1:5 000-1:10 000. IF: 1:5 000

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Datasheet

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Link to on EnCor’s website

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References :

1. Nogales E. Structural insight into microtubule function. Annu Rev Biophys Biomol Struct 30:397–420 (2001).

2. Perez EA. Microtubule inhibitors: Differentiating tubulin-inhibiting agents based on mechanisms of action, clinical activity, and resistance. Mol Cancer Ther 8:2086-2095 (2009).

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