Neurofilament NF-L Antibody – CPCA-NF-L

Encor Chicken polyclonal to Neurofilament Light chain, NF-L

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found to be particularly abundant in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed as a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, α-internexin, nestin and in some cases vimentin.

Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. To raise this antibody, bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al.(2). Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose in 6M urea followed by preparative SDS-PAGE. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria. The HGNC name for this protein is NEFH.

This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. The resulting polyclonal antibody belongs to the IgY subclass. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G. The IgY preparation was made by chloroform delipidation of egg yolk followed by polyethylene glycol precipitation. The IgY total concentration is is 21.5 mg/ml in phosphate buffered saline plus 10mM sodium azide preservative.

HGNC name(s) : NEFL

Host : Chicken

Clonality : Polyclonal

ID : EnCor Biotechnology Neurofilament NF-L NF-L

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgY

Conjugation : none

Immunogen : Purified porci ne

Mass of detected protein : 68 kDa by SDS-PAGE

Uniprot ID : P07196

KGNC name : NEFL

RRID # : AB_2149931

Purification : Concentrated IgY preparation

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:10 000. IF/ICC and IHC: 1:5 000.

Datasheet

Link to on EnCor’s website

References :

1. Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK and Howland DR. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 336:1268-1277 (2005).

2. Delacourte A, Filliatreau G, Boutteau F, Biserte G and Schrevel J. Study of the 10-nm-filament fraction isolated during the standard microtubule preparation. Biochem J. 191:543-6. (1980).