Mouse TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit – E-EL-M1162

Mouse TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit

The Mouse TFPI ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TFPI. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TFPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TFPI. You can calculate the concentration of TFPI in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Tissue Factor Pathway Inhibitor

Abbreviation : TFPI

Uniprot ID : O54819

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Mouse TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit from Elabscience