Human APOA1 / ApoAI Protein (His tag) – PKSH031486

Recombinant Human APOA1 / ApoAI Protein (His tag)

Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

Synonym(s) : APOA1

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the mature form of human APOA1 (P02647) (Asp25-Gln267) was expressed with a polyhistidine tag at the N-terminus.

Predicted N-terminal amino acid : His

Molecular weight/mass : The recombinant human APOA1 consists of 263 amino acids and predicts a molecular mass of 30.7 KDa. It migrates as an approximately 27-31 KDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the mature form of human APOA1 (P02647) (Asp25-Gln267) was expressed with a polyhistidine tag at the N-terminus.

Formulation : Lyophilized from sterile PBS, 10% Glycerol, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet