Human APEX1 / AP / APEx / Ref-1 Protein (His tag) – PKSH030851

Recombinant Human APEX1 / AP / APEx / Ref-1 Protein (His tag)

The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5′ AP endonuclease, DNA 3′ repair diesterase, 3′-5′ exonuclease and DNA 3′-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.

Synonym(s) : APE , APE1 , APEN , APEX , APX , HAP1 , REF1

Species(s) : Human

Protein Expression Host : E. coli

Protein Construction : A DNA sequence encoding the human APEX1 (P27695) (Pro2-Leu 318) was expressed, with a polyhistidine tag at the N-terminus.

Predicted N-terminal amino acid : Met

Molecular weight/mass : The recombinant human APEX1 consisting of 328 amino acids and migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions as predicted.

Protein Construction : A DNA sequence encoding the human APEX1 (P27695) (Pro2-Leu 318) was expressed, with a polyhistidine tag at the N-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.5

Purity : > 92 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet