Human ALCAM / CD166 Protein (His tag) – PKSH031919

Recombinant Human ALCAM / CD166 Protein (His tag)

Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Synonym(s) : CD166 , FLJ38514 , MEMD , MGC71733 , ALCAM

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the extracellular domain (Met 1-Ala 526) of human ALCAM (NP_001618.2) was expressed, with the C-terminal polyhistidine tag.

Predicted N-terminal amino acid : Trp 28

Molecular weight/mass : The recombinant human ALCAM consists of 510 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, rh ALCAM migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the extracellular domain (Met 1-Ala 526) of human ALCAM (NP_001618.2) was expressed, with the C-terminal polyhistidine tag.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 98 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet