HIS (Histamine) ELISA Kit – E-EL-0032

HIS (Histamine) ELISA Kit

The HIS ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with HIS. During the reaction, Universal HIS in the sample or standard competes with a fixed amount of HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal HIS. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal HIS in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: HIS

Abbreviation : HIS

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,938

Detection Range : 1.563-100 ng/mL

More information about this HIS (Histamine) ELISA Kit from Elabscience