8-epi-PGF2α (8-Epi-Prostaglandin F2 Alpha) ELISA Kit - E-EL-0041

8-epi-PGF2α (8-Epi-Prostaglandin F2 Alpha) ELISA Kit

The 8-epi-PGF2α ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of 8-epi-PGF2α on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: 8-epi-PGF2α

Abbreviation : 15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 9,375

Detection Range : 15.625-1000 pg/mL

More information about this 8-epi-PGF2α (8-Epi-Prostaglandin F2 Alpha) ELISA Kit from Elabscience