EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix Comparison with competitor RT kits and mixes
AE311EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix
AE311-02 199$/50 rx
- cDNA lenght : ≤ 8 kb
- Detection limit: 50 ng total RNA
- Reaction Temp. : 42 °C
- gDNA removal: Included & Simultaneous (not a separate step from the RT rx)
- RNase H activity: No
- Oligos included: Anchored Oligo(dT)18 and Random Primers (100$ value)
- RNase inhibitor included: Yes
- Note: Available without gDNA removal – TGAE301-02 for 174$
Omniscript RT Kit
205111 272$/50 rx
- cDNA lenght : ≤ 8 kb
- Detection limit: 50 ng total RNA
- Reaction Temp. : 42 °C
- gDNA removal: separate step | requires buying DNase I
- RNase H activity: Yes
- Oligos included: none
- RNase inhibitor included: No
ProtoScript® First Strand cDNA Synthesis Kit
E6300S 308$/50 rx (185$/30 rx)
- cDNA lenght : ≤ 10 kb
- Detection limit: 1 ng total RNA
- Reaction Temp. : 42 °C
- gDNA removal: separate step | requires buying DNase I
- RNase H activity: Yes
- Oligos included: Oligo d(T)23 VN and Random primers
- RNase inhibitor included: Yes
High-Capacity cDNA Reverse Transcription Kit
4368814 59$/50 rx (236$/200rx)
- cDNA lenght : ≤ 7 kb
- Detection limit: 20 ng total RNA
- Reaction Temp. : 37 °C
- gDNA removal: not inluded | requires buying DNase I
- RNase H activity: Yes
- Oligos included: Random primers only
- RNase inhibitor included: No
- Notes: Not a mastermix | low yield
EasyScript® Supermix Comparison with other Transgen Biotech RT Supermixes
EasyScript® vs TransScriptScript® vs TransScript® II RT vs FLY vs Uni SupermixesEasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix
AE311-02 199$/50 rx
- cDNA lenght : ≤ 8 kb
- Detection limit: 50 ng total RNA
- Reaction Temp. : 42 °C
- gDNA removal: Included & Simultaneous (not a separate step from the RT rx)
- RNase H activity: No
- Oligos included: Anchored Oligo(dT)18 and Random Primers (100$ value)
- RNase inhibitor included: Yes
- Note: Available without gDNA removal – AE301-02 for 174$
TransScript® One-Step gDNA Removal and cDNA Synthesis SuperMix
AT311-02 285$/50 rx
- cDNA lenght : ≤ 12 kb
- Detection limit: 1 pg
- Reaction Temp. : 42 °C
- gDNA removal: Included & Simultaneous (not a separate step from the RT rx)
- RNase H activity: No
- Oligos included: Anchored Oligo(dT)18 and Random Primers (100$ value)
- RNase inhibitor included: Yes
- Note: Available without gDNA removal – AT301-02 for 242$
TransScript® II One-Step gDNA Removal and cDNA Synthesis SuperMix
AH311-02 495$/50 rx
- cDNA lenght : ≤ 15 kb
- Detection limit: 1 pg
- Reaction Temp. : 42-55 °C
- gDNA removal: Included & Simultaneous (not a separate step from the RT rx)
- RNase H activity: No
- Oligos included: Anchored Oligo(dT)20 and Random Primers (100$ value)
- RNase inhibitor included: Yes
- Note: Available without gDNA removal – AH301-02 for 433$
TransScript® FLY (5 min) First-Strand cDNA Synthesis SuperMix
AF301-02 297$/50 rx
- cDNA lenght : ≤ 12 kb
- Detection limit: 1 pg
- Reaction Temp. : 42 °C
- gDNA removal: not included | requires buying DNase I
- RNase H activity: No
- Oligos included: Anchored Oligo(dT)18 and Random Primers (100$ value)
- RNase inhibitor included: Yes
- Note: 5 min cDNA synthesis (12 kb)
TransScript® -Uni One-Step gDNA Removal and cDNA Synthesis SuperMix
AU311-02 564$/50 rx
- cDNA lenght : ≤ 20 kb
- Detection limit: < 1 pg
- Reaction Temp. : 42-65 °C
- gDNA removal: Included & Simultaneous (not a separate step from the RT rx)
- RNase H activity: No
- Oligos included: Anchored Oligo(dT)20 and Random Primers (100$ value)
- RNase inhibitor included: Yes