VA (Vitamin A) ELISA Kit – E-EL-0006

VA (Vitamin A) ELISA Kit

The VA ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with VA. During the reaction, Universal VA in the sample or standard competes with a fixed amount of VA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VA. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal VA in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: VA

Abbreviation : ATRA, Retinol, β-Carotene, Beta-Carotene, Tretinoin, All-Trans Retinoic Acid, α-Carotene, Alpha-Carotene

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,094

Detection Range : 0.156-10 ng/mL

More information about this VA (Vitamin A) ELISA Kit from Elabscience