Rat TXB3 (Thromboxanes B3) ELISA Kit – E-EL-R0966

Rat TXB3 (Thromboxanes B3) ELISA Kit

The TXB3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TXB3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TXB3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TXB3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TXB3. You can calculate the concentration of Rat TXB3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Thromboxanes B3

Abbreviation : TXB3

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Rat TXB3 (Thromboxanes B3) ELISA Kit from Elabscience