Rat TRH (Thyrotropin-Releasing Hormone) ELISA Kit – E-EL-R0979

Rat TRH (Thyrotropin-Releasing Hormone) ELISA Kit

The TRH ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with TRH. During the reaction, Rat TRH in the sample or standard competes with a fixed amount of TRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat TRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat TRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Thyrotropin-Releasing Hormone

Abbreviation : TRH

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

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