Rat TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit – E-EL-R1041

Rat TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

The TRAIL/TNFSF10 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TRAIL/TNFSF10. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TRAIL/TNFSF10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TRAIL/TNFSF10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TRAIL/TNFSF10. You can calculate the concentration of TRAIL/TNFSF10 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Tumor Necrosis Factor Related Apoptosis Inducing Ligand

Abbreviation : TRAIL/TNFSF10

Uniprot ID : Q8K3G0

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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