Rat TMED1 (Transmembrane Emp24 Domain Containing Protein 1) ELISA Kit – E-EL-R1020

Rat TMED1 (Transmembrane Emp24 Domain Containing Protein 1) ELISA Kit

The TMED1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TMED1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TMED1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TMED1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TMED1. You can calculate the concentration of Rat TMED1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Transmembrane Emp24 Domain Containing Protein 1

Abbreviation : TMED1

Uniprot ID : Q5BK85

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Rat TMED1 (Transmembrane Emp24 Domain Containing Protein 1) ELISA Kit from Elabscience