Rat TDGF1 (Teratocarcinoma Derived Growth Factor 1) ELISA Kit – E-EL-R0950

Rat TDGF1 (Teratocarcinoma Derived Growth Factor 1) ELISA Kit

The TDGF1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TDGF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TDGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TDGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TDGF1. You can calculate the concentration of TDGF1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Teratocarcinoma Derived Growth Factor 1

Abbreviation : TDGF1

Uniprot ID : F1M2P2

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

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