Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit – E-EL-R0951

Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit

The TCCC5b-9 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TCC C5b-9. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TCC C5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TCC C5b-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TCC C5b-9. You can calculate the concentration of TCC C5b-9 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Terminal Complement Complex C5b-9

Abbreviation : TCCC5b-9

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Rat TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit from Elabscience