Rat SPLA2 (Secreted Phospholipase A2) ELISA Kit – E-EL-R0873

Rat SPLA2 (Secreted Phospholipase A2) ELISA Kit

The SPLA2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to sPLA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for sPLA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain sPLA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of sPLA2. You can calculate the concentration of sPLA2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Secreted Phospholipase A2

Abbreviation : SPLA2

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 3.75 pg/ml

Detection Range : 6.25 – 400 pg/ml

Manual & Datasheet

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