Rat TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit
The TAFI ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TAFI. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TAFI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TAFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TAFI. You can calculate the concentration of TAFI in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Thrombin Activatable Fibrinolysis Inhibitor
Abbreviation : TAFI
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 0.938 ng/ml
Detection Range : 1.563 – 100 ng/ml
Additional information
Format | 96 rx |
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