Rat SYP (Synaptophysin) ELISA Kit – E-EL-R0932

Rat SYP (Synaptophysin) ELISA Kit

The SYP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SYP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for SYP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SYP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SYP. You can calculate the concentration of SYP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Synaptophysin

Abbreviation : SYP

Uniprot ID : P07825

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.375 ng/ml

Detection Range : 0.625 – 40 ng/ml

Manual & Datasheet

More information about this Rat SYP (Synaptophysin) ELISA Kit from Elabscience