Rat STAT5B (Signal Transducer And Activator Of Transcription 5B) ELISA Kit – E-EL-R0884

Rat STAT5B (Signal Transducer And Activator Of Transcription 5B) ELISA Kit

The STAT5B ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat STAT5B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat STAT5B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat STAT5B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat STAT5B. You can calculate the concentration of Rat STAT5B in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Signal Transducer And Activator Of Transcription 5B

Abbreviation : STAT5B

Uniprot ID : P52632

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Rat STAT5B (Signal Transducer And Activator Of Transcription 5B) ELISA Kit from Elabscience