Rat SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit – E-EL-R0921

Rat SREBP (Sterol Regulatory Element Binding Proteins) ELISA Kit

The SREBP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SREBP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for SREBP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SREBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SREBP. You can calculate the concentration of SREBP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Sterol Regulatory Element Binding Proteins

Abbreviation : SREBP

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.234 ng/ml

Detection Range : 0.391 – 25 ng/ml

Manual & Datasheet

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