Rat ON (Osteonectin) ELISA Kit – E-EL-R0701

Rat ON (Osteonectin) ELISA Kit

The ON ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ON. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ON and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ON, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ON. You can calculate the concentration of ON in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Osteonectin

Abbreviation : ON

Uniprot ID : P16975

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Rat ON (Osteonectin) ELISA Kit from Elabscience