Rat LAG3 / CD223 / Lymphocyte activation gene 3 Protein (His tag) – PKSR030201

Recombinant Rat LAG3 / CD223 / Lymphocyte activation gene 3 Protein (His tag)

LAG3, also known as CD223 and Lymphocyte activation gene 3, belongs to immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells. It also acts as as a new marker of T cell induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signalling, leading to increased antigen-specific T-cell responses in vivo.

Synonym(s) : LAG3

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat LAG3 (Q5BK54)(Met1-Leu442) was expressed with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Gly 24

Molecular weight/mass : The recombinant rat LAG3 comprises 430 amino acids and predicts a molecular mass of 47.1 kDa. The apparent molecular mass of the recombinant protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Construction : A DNA sequence encoding the rat LAG3 (Q5BK54)(Met1-Leu442) was expressed with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile 20 mM NaAc, 500 mM NaCl, 10 % Glycerol, 0.02 % tween 20, pH 5.5

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet