Rat IL4R / Il4ra Protein (Fc tag) – PKSR030323

Recombinant Rat IL4R / Il4ra Protein (Fc tag)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells.

Synonym(s) : IL4R , Il4ra

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat IL4R (Q63257-1) (Met1-Arg232) was expressed with the Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Ile 26

Molecular weight/mass : The recombinant rat IL4R/Fc is a disulfide-linked homodimer. The reduced monomer comprises 448 amino acids and has a predicted molecular mass of 50.9 kDa. The apparent molecular mass of the protein is approximately 66 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the rat IL4R (Q63257-1) (Met1-Arg232) was expressed with the Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet