Rat HER2 / ErbB2 Protein – PKSR030371

Recombinant Rat HER2 / ErbB2 Protein

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Synonym(s) : ErbB2 , Neu , p185erbB2

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat ErbB2 (AAH61863.1) extracellular domain (Met 4-Thr 656) was expressed and purified, with seven additional amino acids (ALEVLFQ) at the C-terminus.

Predicted N-terminal amino acid : Thr 26

Molecular weight/mass : The recombinant rat ErbB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 638 amino acids and predicts a molecular mass of 70.6 kDa. The apparent molecular mass of the rat ErbB2 is approximately 95-105 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Construction : A DNA sequence encoding the rat ErbB2 (AAH61863.1) extracellular domain (Met 4-Thr 656) was expressed and purified, with seven additional amino acids (ALEVLFQ) at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 92 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet