Rat EpCAM / TROP-1 / TACSTD1 Protein (His tag) – PKSR030246

Recombinant Rat EpCAM / TROP-1 / TACSTD1 Protein (His tag)

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Synonym(s) : EPCAM , Tacstd1

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat EPCAM (O55159) (Met1-Thr266) was expressed, fused with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Gln 24

Molecular weight/mass : The recombinant rat EPCAM comprises 254 amino acids and predicts a molecular mass of 29.2 kDa. The apparent molecular mass of the recombinant protein is approximately 39 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Protein Construction : A DNA sequence encoding the rat EPCAM (O55159) (Met1-Thr266) was expressed, fused with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 97 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet