Rat AP13 (Apelin 13) ELISA Kit – E-EL-R2574

Rat AP13 (Apelin 13) ELISA Kit

The AP13 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Rat AP13. During the reaction, Rat AP13 in the sample or standard competes with a fixed amount of Rat AP13 on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Rat AP13. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat AP13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Apelin 13

Abbreviation : AP13

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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