Rat ADAMTS13/VWFCP (Von Willebrand Factor Cleaving Protease) ELISA Kit – E-EL-R1078

Rat ADAMTS13/VWFCP (Von Willebrand Factor Cleaving Protease) ELISA Kit

The ADAMTS13/VWFCP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADAMTS13/VWFCP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ADAMTS13/VWFCP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADAMTS13/VWFCP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ADAMTS13/VWFCP. You can calculate the concentration of Rat ADAMTS13/VWFCP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Von Willebrand Factor Cleaving Protease

Abbreviation : ADAMTS13/VWFCP

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

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