Rat δSIP (Delta Sleep Inducing Peptide ) ELISA Kit
The Rat δSIP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Rat δSIP. During the reaction, Rat δSIP in the sample or standard competes with a fixed amount of Rat δSIP on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Rat δSIP. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat δSIP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<
Detected Target name: Rat Delta Sleep Inducing Peptide
Abbreviation : Rat δSIP
Format : 96 rx
Reactivity : Rat
ELISA configuration : Competitive
Sensitivity : 0.094 ng/ml
Detection Range : 0.156 – 10 ng/ml
More information about this Rat δSIP (Delta Sleep Inducing Peptide ) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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