Rat α1-AT (Alpha 1-Antitrypsin) ELISA Kit – E-EL-R2420

Rat α1-AT (Alpha 1-Antitrypsin) ELISA Kit

The α1-AT ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to α1-AT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for α1-AT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain α1-AT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of α1-AT. You can calculate the concentration of α1-AT in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Alpha 1-Antitrypsin

Abbreviation : α1-AT

Uniprot ID : P17475

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 3.75 ng/ml

Detection Range : 6.25 – 400 ng/ml

Manual & Datasheet

More information about this Rat α1-AT (Alpha 1-Antitrypsin) ELISA Kit from Elabscience