PerfectStart Green qPCR SuperMix (SYBR green) – AQ601
Diagnostic-grade qPCR with < 1 copy E.Coli gDNA
$97.00 – $1,192.00 CAD
PerfectStart Green qPCR SuperMix
PerfectStart™ Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a PerfectStart™ Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer and PCR stabilizer. PerfectStart Taq DNA polymerase is a hot-start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, passive reference dye (optional) and nuclease-free water.
PerfectStart Green qPCR SuperMix is also available ain 5x and 10x formulations (special request)
Features of PerfectStart Green qPCR SuperMix
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PerfectStart Taq DNA Polymerase enables high specificity, high sensitivity, high amplification efficiency.
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Dual-cation buffer enhances specificity and reduces primer-dimer formation.
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Passive reference dyes (to normalize tube-to-tube differences due to pipetting errors) are provided for different qPCR instruments.
Kit Content
- 2x PerfectStart Green qPCR SuperMix
- Passive Reference Dye I and II (50x)
- ddH2O
Reference Dye compatibility
- Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus - Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 - No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q
Manual and Datasheet
AQ601 – PerfectStart Green qPCR SuperMix datasheet
Additional information
| Format | 1 ml (100 rx), 5 x 1ml (500 rx), 15 x 1 ml (1 500 rx), 25 x 1 ml (2 500 rx) |
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| Supplier |
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Gabriela Galicia (verified owner) –
I used PerfectStart supermix to evaluate an shRNA knockdown in human AML cell lines. I used the two step qPCR protocol with FastABI systems and it worked very well. I tested PerfectStart mix with two set of primers for the same gene and I got very good reproducibility and similar results comparing both primer sets.
Simon Roy –
Thanks Gaby! Here’s your code for a 25$ discount on your next purchase 🙂
cb-gabAQ601
Simon