PerfectStart Green qPCR SuperMix (SYBR green) – AQ601

PerfectStart Green qPCR SuperMix

PerfectStart™ Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a PerfectStart™ Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer and PCR stabilizer. PerfectStart Taq DNA polymerase is a hot-start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, passive reference dye (optional) and nuclease-free water.

PerfectStart Green qPCR SuperMix is also available ain 5x and 10x formulations (special request)

Features of PerfectStart Green qPCR SuperMix

• PerfectStart^TM Taq DNA Polymerase enables high specificity, high sensitivity, high amplification efficiency.

• Dual-cation buffer enhances specificity and reduces primer-dimer formation.

• Passive reference dyes (to normalize tube-to-tube differences due to pipetting errors) are provided for different qPCR instruments.

Kit Content

• 2x PerfectStart Green qPCR SuperMix
• Passive Reference Dye I and II (50x)
• ddH2O

Reference Dye compatibility

• Passive Reference Dye I (50×)
  ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
  ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
  Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

Manual and Datasheet

AQ601 – PerfectStart Green qPCR SuperMix datasheet