PerfectStart Green qPCR SuperMix (SYBR green) – AQ601
Diagnostic-grade qPCR with < 1 copy E.Coli gDNA
97.00$ – 1,392.00$CAD
PerfectStart Green qPCR SuperMix
PerfectStart™ Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a PerfectStart™ Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer and PCR stabilizer. PerfectStart Taq DNA polymerase is a hot-start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, passive reference dye (optional) and nuclease-free water.
PerfectStart Green qPCR SuperMix is also available ain 5x and 10x formulations (special request)
Features of PerfectStart Green qPCR SuperMix
PerfectStartTMTaq DNA Polymerase enables high specificity, high sensitivity, high amplification efficiency.
Dual-cation buffer enhances specificity and reduces primer-dimer formation.
Passive reference dyes (to normalize tube-to-tube differences due to pipetting errors) are provided for different qPCR instruments.
Kit Content
- 2x PerfectStart Green qPCR SuperMix
- Passive Reference Dye I and II (50x)
- ddH2O
Reference Dye compatibility
- Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus - Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000 - No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q
Manual and Datasheet
AQ601 – PerfectStart Green qPCR SuperMix datasheet
Additional information
| Format | 1 ml (100 rx), 5 x 1ml (500 rx), 15 x 1 ml (1 500 rx), 25 x 1 ml (2 500 rx) |
|---|---|
| Supplier |
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1 review for PerfectStart Green qPCR SuperMix (SYBR green) - AQ601
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Gabriela Galicia (verified owner)–
I used PerfectStart supermix to evaluate an shRNA knockdown in human AML cell lines. I used the two step qPCR protocol with FastABI systems and it worked very well. I tested PerfectStart mix with two set of primers for the same gene and I got very good reproducibility and similar results comparing both primer sets.
Simon Roy –
Thanks Gaby! Here’s your code for a 25$ discount on your next purchase 🙂
cb-gabAQ601
Simon