Neurofilament NF-H – Phosphate independent Antibody – RPCA-NF-H-Pind

Encor Rabbit Polyclonal to Neurofilament NF-H (phosphate independent, non-phospho)

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant, biochemically stable. The form of NF-H expressed in neurons varies in terms of the level of phosphorylation.
Mammalian NF-H contains tandem repeats based on the sequence lysine serine proline (KSP), which may be present up to 60 times, with some variability between species. These KSP sequences are the sites of phosphorylation, and in axons most if not all of the serine residues are phosphorylated. In contrast, neurofilaments in dendrites and perikarya are not phosphorylated on these KSP sequences. Axonal neurofilaments are therefore quite different immunologically from those in perikarya and dendrites, and many antibodies stain only axonal or only dendritic/perikaryal neurofilaments.

This antibody was raised against the KSP region of rat NF-H which was expressed in and purified from E. coli. It is similar to, but higher quality than, an antibody made and described many years ago (1). Since bacteria do not phosphorylate mammalian proteins, this protein resembles the NF-H form characteristic of perikaryal and dendritic neurofilaments. Accordingly this antibody stains dendritic and perikaryal neurofilaments particularly well.
The HGNC name for this protein is NEFH.

HGNC name(s) : NEFH

Host : Rabbit

Clonality : Polyclonal

ID : EnCor Biotechnology Neurofilament NF-H – Phosphate independent NF-H-Pind

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG

Conjugation : none

Immunogen : Recombinant construct with most of the tandem KSP repeats

Mass of detected protein : 200 kDa

Uniprot ID : P12036

KGNC name : NEFH

RRID # : AB_2572361

Purification : Serum

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:10 000. ICC/IF and IHC: 1:1 000.

Datasheet

Link to on EnCor’s website

References :

1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.