Mouse TREM-1 (Triggering Receptor Expresses on Myeloid Cells-1) ELISA Kit – E-EL-M0645

Mouse TREM-1 (Triggering Receptor Expresses on Myeloid Cells-1) ELISA Kit

The Mouse TREM-1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TREM-1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TREM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TREM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TREM-1. You can calculate the concentration of TREM-1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Triggering Receptor Expresses on Myeloid Cells-1

Abbreviation : TREM-1

Uniprot ID : Q9JKE2

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

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