Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) ELISA Kit – E-EL-M1203

Mouse TNNI3/cTn-I (Troponin I Type 3, Cardiac) ELISA Kit

The Mouse TNNI3/cTn-I ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNNI3/cTn-I. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TNNI3/cTn-I. You can calculate the concentration of TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Troponin I Type 3, Cardiac

Abbreviation : TNNI3/cTn-I

Uniprot ID : P48787

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 4.688 pg/ml

Detection Range : 7.813 – 500 pg/ml

Manual & Datasheet

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