Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit – E-EL-M0049

Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit

The Mouse TNF-α ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNF-α. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TNF-α. You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Tumor Necrosis Factor Alpha

Abbreviation : TNF-α

Uniprot ID : P06804

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit from Elabscience