Mouse C3 (Complement 3) ELISA Kit – E-EL-M0330

Mouse C3 (Complement 3) ELISA Kit

The Mouse C3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for C3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of C3. You can calculate the concentration of C3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Complement 3

Abbreviation : C3

Uniprot ID : P01027

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 1.875 ng/ml

Detection Range : 3.125 – 200 ng/ml

Manual & Datasheet

More information about this Mouse C3 (Complement 3) ELISA Kit from Elabscience