Mouse AXIN2 (Axis Inhibition Protein 2) ELISA Kit – E-EL-M0167

Mouse AXIN2 (Axis Inhibition Protein 2) ELISA Kit

The Mouse AXIN2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AXIN2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AXIN2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AXIN2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AXIN2. You can calculate the concentration of AXIN2 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Axis Inhibition Protein 2

Abbreviation : AXIN2

Uniprot ID : O88566 

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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