Mouse ASK1 (Apoptosis Signal Regulating Kinase 1) ELISA Kit – E-EL-M0142

Mouse ASK1 (Apoptosis Signal Regulating Kinase 1) ELISA Kit

The Mouse ASK1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ASK1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ASK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ASK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ASK1. You can calculate the concentration of ASK1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Apoptosis Signal Regulating Kinase 1

Abbreviation : ASK1

Uniprot ID : O35099 

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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