Human IL-28B / IFN-lambda-3 Protein (His Tag) – PKSH030978

Recombinant Human IL-28B / IFN-lambda-3 Protein (His Tag)

Interleukin-28B (IL-28B) also known as Interferon lambda-3 and IFN-lambda-3, belongs to the type III interferon family of cytokines and are highly similar to IL-29. IL-28B belongs to the newly described interferon lambda (IFNλ) family of cytokines. IL-28B is a cytokine with immunomodulatory activity. It functions in Up-regulating MHC class I antigen expression. IL-28B displays potent antiviral activity and antitumor activity. This cytokine serves as ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, is able to increase the percentage of splenic CD8+ T cells in vaccinated animals, and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant.

Synonym(s) : IL28B , IL28C

Species(s) : Human

Protein Expression Host : Yeast

Protein Construction : A DNA sequence encoding the human IFNL3 (NP_742151.2) (Val18-Val196) was expressed with a polyhistidine tag at the C-terminus.

Predicted N-terminal amino acid : Val 18

Molecular weight/mass : The recombinant human IFNL3 consists 189 amino acids and predicts a molecular mass of 21.3 kDa.

Protein Construction : A DNA sequence encoding the human IFNL3 (NP_742151.2) (Val18-Val196) was expressed with a polyhistidine tag at the C-terminus.

Formulation : Lyophilized from sterile PBS, PH 7.4

Purity : > 95 % as determined by SDS-PAGE.

Endotoxin level :

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet