Human HGF (Hepatocyte Growth Factor) ELISA Kit – E-EL-H0084

Human HGF (Hepatocyte Growth Factor) ELISA Kit

The Human HGF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HGF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for HGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HGF. You can calculate the concentration of HGF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Hepatocyte Growth Factor

Abbreviation : HGF

Uniprot ID : P14210

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 75 pg/ml

Detection Range : 125 – 8000 pg/ml

Manual & Datasheet

More information about this Human HGF (Hepatocyte Growth Factor) ELISA Kit from Elabscience