Human ADP/Acrp30 (Adiponectin) ELISA Kit – E-EL-H0004

Human ADP/Acrp30 (Adiponectin) ELISA Kit

The Human ADP/Acrp30 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ADP/Acrp30. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ADP/Acrp30. You can calculate the concentration of ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Adiponectin

Abbreviation : ADP/Acrp30

Uniprot ID : Q15848

Format : 96 rx

Reactivity : Human

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

More information about this Human ADP/Acrp30 (Adiponectin) ELISA Kit from Elabscience